Getting enough protein

It utilises the SEQUEST algorithm that will search protein databases and now features probability- based scoring to complement more traditional SEQUEST scores. A new unified search-results file format to speed up data analysis, as well as support for accurate mass searching, is also part of this new package. The XPRESS stable-isotope quantitation algorithm and the protein area/height calculation have been improved. The application also provides more support to new instruments such as the Finnigan vMALDI-equipped mass spectrometers. BioWorks identifies proteins by comparing experimental tandem mass spectrometry (MS/MS) data with standard protein and DNA databases. It can analyse a single spectrum or an entire LC/MS/MS peptide map. And a powerful advantage of the SEQUEST algorithm is an ability to analyse proteins in mixtures. This algorithm can identify low-concentration proteins generating a weak signal. The XPRESS algorithm automates quantitative protein expression analysis, simplifying interpretation of data from differential labelling techniques such as the ICAT reagent method.