High-resolution phase contrast and fluorescence imaging is now possible using the same objective, thanks to the special external phase contrast system on NikonÆs new Ti series inverted microscopes. Furthermore, this flexibility is now achieved with maximum light transmission.
The Ti series exclusive use of high NA objectives without phase rings to achieve phase contrast enables the capture of brighter fluorescence images, as essentially they recapturing the 10–15% of light traditionally blocked out by the phase annulus in the objective. In addition, the external phase contrast unit minimises aberration and double image acquisition to achieve crisp cellular definition.
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