Concentrated total RNA from extremely small samples

Based on reliable silica membrane technology, the innovative column design of the NucleoSpin RNA XS requires elution volumes of only 5–20 ìL. Combined with higher RNA yields, this small elution volume results in a highly concentrated, highly purified sample that is ideal for sensitive downstream applications such as RT-PCR.

NucleoSpin RNA XS is suitable for use with extremely small samples (0.1 mg tissue or one cell) or with larger sample sizes (up to 5 mg tissue or 100 cells). Optimal RNA yield is ensured by the inclusion of chaotropic ions within the lysis buffer that inactivate RNases (present in virtually all biological materials), thus preventing degradation of the RNA during the isolation procedure.

In addition, the kit offers the timesaving option of on-column removal of contaminating DNA using an RNase-free recombinant DNase, which is supplied with the kit. The purified RNA is eluted under low ionic strength conditions in as little as 5 ìL RNase-free water (also supplied).

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