Integrated DNA Technologies (IDT) has added additional flexibility to its PrimeTime quantitative polymerase chain reaction (qPCR) product. Users can now customise the Standard and XL qPCR assays by specifying the dye and quencher pair used on the probe and the ratio of primer to probe.
These new PrimeTime features work in combination with the existing versatility offered across the PrimeTime range, including the ability to set the design parameters, select exon spanning sites to reduce genomic contamination, and view the primer and probe sequences prior to purchase.
PrimeTime qPCR assays are 5’-nuclease assays that consist of a forward and reverse primer together with a dual-labelled probe. The assays are shipped lyophilised with all components in a single tube, with enough reagents for 500 (Standard) or 2500 (XL) reactions.
The use of a fluorescent probe eliminates inaccuracies associated with primer dimers or non-specific binding. Fluorescence is detected only when the probe is cleaved by the polymerase action of the elongation phase. This improved specificity ensures precise and reproducible results with every reaction.
www.idtdna.com/primetime