Multiphoton optics for deeper tissue imaging

The 25x Olympus 4 mm (NA 1.0) and 8 mm (NA 0.9) SCALEVIEW microscope objectives allow researchers to create highly accurate 3D structural representations of tissue from intact specimens by offering detailed, crisp images over superlong working distances. Using a combination of the cutting edge FluoView FV1000MPE multiphoton microscope system, SCALEVIEW optics and a reagent developed by Dr Atsushi Miyawaki in Japan, which literally makes tissue transparent (Hama H, Kurokawa H, Kawano H et al. Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nat Neurosci 2011; 14 [11]: 1481–8), researchers can see up to 8 mm into the tissue without the need for sectioning.
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