Oxford Gene Technology (OGT) has made several advances in hybridisation-based target enrichment protocols that now enable researchers to prepare samples for sequencing in just one day. Researchers can now access the high-quality results of hybridisation-based targeted sequencing with a speed comparable to that of amplicon-based approaches.
Oxford Gene Technology’s hybridisation-based targeted sequencing delivers unparalleled coverage uniformity enabling researchers to detect low-frequency variants consistently down to 1% variant allele frequency (VAF) at a read depth of >1000 times. The company’s latest protocol developments enable researchers to focus quickly and precisely in on regions relevant to their research - increasing throughput and confidence in results while saving time and reagents.
The company’s hybridisation assay enhancements improve speed and also enable lower input levels of DNA to be used, and optimised enzymes and buffers allow pooling of library preparation steps, enabling users to complete the whole enrichment protocol in less time. This includes a short enzymatic fragmentation step, combined end-repair and adaptor ligation steps, and an optimised hybridisation of just 30 minutes for good-quality DNA samples. In addition, OGT’s next-generation sequencing (NGS) panel optimisation yields good data with as little as 10 ng of starting material.
The company has also recently expanded its fully tested and optimised SureSeq myPanel NGS Custom Cancer Panel content, covering both haematology and solid tumours. The new content provides optimised coverage for over 90 genes, including difficult-to-sequence genes and other regions.