Double staining streamlines Immunofluorescence Workflow

Vector Laboratories has introduced the VectaFluor Duet kit, which offers convenient, ready-to-use immunofluorescence double labelling. This new kit is ideal for use on a wide range of frozen and formalin-fixed, paraffin wax-embedded (FFPE) tissue samples, combining primary and secondary antibodies to streamline workflow. VectaFluor Duet includes a secondary reagent to detect mouse and rat primary antibodies with green and red DyLight fluorescent dyes and a normal blocking serum. With the kit, one protocol replaces several and reduces preparation time, in some cases by as much as a day or more.

“These two reagents are commonly used together,” explains Harjit Kullar (Maravai LifeSciences). “Using two stains sequentially requires a tedious, time-consuming protocol and complex workflow. The VectaFluor Duet combines the double staining into one protocol, providing time and cost savings.”

In the new kit, Vector Laboratories’ affinity-purified, cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a process that ensures maximum labelling without compromising antibody affinity or specificity. The red and green DyLight anti-mouse/anti-rabbit antibodies are combined into a robust, stable cocktail that yields sensitive and consistent dual staining. 

 

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