New RNase Inhibitor ensures reliable RNA protection
PCR Biosystems has launched RiboShield RNase Inhibitor, a robust and reliable RNase inhibitor that is now available as a standalone product.
RiboShield RNase Inhibitor is a recombinant protein that blocks the activity of a wide range of ribonucleases to protect RNA from RNase digestion. The inhibitor is designed for use in various RNA-sensitive applications including RNA purification, complementary DNA (cDNA) synthesis, RT-PCR, RNA sequencing, and in situ hybridisation.
With such techniques, the presence of even small amounts of RNase can be highly detrimental to RNA quality and experimental outcome. RiboShield RNase Inhibitor also complements PCR Biosystems’s range of products to support SARS-CoV-2 research and testing.
One new application for RiboShield RNase Inhibitor is in saliva-based testing for the presence of SARS-CoV-2. This is currently gaining popularity as it is easy for people to collect their own samples with minimal discomfort compared with the standard nasopharyngeal swab. While the sample is simple to obtain, the digestive enzymes present in saliva represent a hostile environment for RNA. Using an RNase inhibitor is essential to provide adequate RNA protection and generate accurate test results.
RiboShield RNase Inhibitor performs over a wide range of reaction conditions and can inhibit RNases at temperatures up to 65°C for at least 30 minutes. The molecule binds non-covalently at a ratio of 1:1 to inhibit the activity of various ribonucleases, including eukaryotic RNases of the neutral type (RNases A, B and C). The inhibitor does not hinder other enzymes, such as reverse transcriptases, RNA polymerases or Taq DNA polymerase, making it compatible with many enzymatic reactions that utilise RNA.
In addition, the very rapid kinetics of association to RNases guarantees immediate protection of RNA, leading to significantly better performance in applications where RNase contamination is a concern. The RiboShield RNase Inhibitor protein is purified from a strain of Pichia pastoris that expresses a modified human placental gene.
More information on RiboShield and the complete range of products to support RNA analysis and SARS-CoV-2 testing is available here.
Alternatively, to request a free sample, visit https://pcrbio.com/quotes-samples/?_product=7712,