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New nasal vaccine shows promise for COVID-19

New research shows that a needle-free mucosal bacteriophage (phage) T4-based COVID-19 vaccine is effective against SARS-CoV-2 infection.

The findings were published in mBio, an open access journal of the American Society for Microbiology.

In recent years, the Food and Drug Administration authorised mRNA- and adenovirus-based SARS-CoV-2 vaccines. These vaccines are intramuscularly injected in two or more doses and are effective in preventing COVID-19, but they do not induce efficient mucosal immunity or prevent viral transmission.

In the new study, senior study authors Venigalla B Rao, PhD, from the Bacteriophage Medical Research Center, Department of Biology, The Catholic University of America, Washington, DC, and Ashok K Chopra, PhD, CSc, Department of Microbiology & Immunology, The University of Texas Medical Branch, Galveston, Texas, and their colleagues report the first non-infectious, bacteriophage T4-based, multicomponent, needle and adjuvant-free mucosal vaccine. Both of the senior authors are elected fellows of the American Academy of Microbiology.

In experiments conducted in mice, intranasal administration of two doses of the phage T4-COVID-19 vaccine 21-days apart induced robust mucosal immunity, in addition to strong systemic humoral and cellular immune responses. The intranasal vaccine induced broad virus neutralisation antibody titers against multiple variants and triggered Th1-biased cytokine responses, strong CD4+ and CD8+ T cell immunity, and high secretory IgA titers in sera and bronchoalveolar lavage of vaccinated mice. All these responses were much stronger in intranasally vaccinated mice than that induced by the injected vaccine. Furthermore, the nasal vaccine provided complete protection and sterilising immunity against the mouse-adapted SARS-CoV-2 MA10 strain, the ancestral WA-1/2020 strain, and the most lethal Delta variant in mouse models.

Additionally, the T4-COVID-19 vaccine elicited broad virus-neutralising antibodies against SARS-CoV-2 variants in sera and bronchoalveolar lavage, did not affect the gut microbiota, exhibited minimal lung lesions in vaccinated and challenged mice and is stable at ambient temperature.

“This intranasally administered vaccine generates superior mucosal immunity in mice in addition to inducing robust humoral and cell-mediated immune responses, and provides complete protection and sterilising immunity against SARS-CoV-2 variants. The vaccine is stable, adjuvant-free and cost-effectively manufactured and distributed, making it a strategically important next-generation COVID-19 vaccine for ending this pandemic,” said Drs Rao and Chopra. “This modular, needle-free, phage T4 mucosal vaccine delivery platform is an excellent candidate to design efficacious mucosal vaccines against other respiratory infections and for emergency preparedness against emerging epidemic and pandemic pathogens.”

The full paper is available to read at the link below.

https://journals.asm.org/doi/10.1128/mbio.01822-22?_ga=2.63271343.1836060417.1659084836-1084905913.1659084836

 

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Upcoming Events

Transfusing Wisely: a free-to-access SHOT webinar

Online
6 December 2022

Microbiology Society Annual Conference 2023

Birmingham International Convention Centre.
17-20 April 2023

British Society for Microbial Technology Annual Microbiology Conference

Royal Air Force Museum, Hendon, London
10 May 2023

Microscience Microscopy Congress 2023 (mmc 2023, incorporating EMAG 2023)

Manchester Central Conference Centre
4-6 July 2023

IBMS Congress 2023

ICC Birmingham
25-28 September 2023

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

Download the FREE Pathology In Practice app from your device's App store

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