Biotium has announced the release of its latest product, Mix-n-Stain CF Dye IgM Antibody Labelling Kits. These kits are able to enhance the labelling process for IgM antibodies, enabling researchers to easily obtain high-performance fluorescent IgM conjugates with minimal hands-on time and no purification after labelling.
IgM antibodies offer advantages over IgG antibodies due to their increased avidity. However, traditional methods for conjugation of IgG antibodies are frequently ineffective for optimal labelling of IgM antibodies due to differences in the immunoglobulin structures. Recognising this roadblock, Biotium has successfully optimised the reaction conditions and developed Mix-n-Stain CF Dye IgM Antibody Labelling Kits to address this issue.
With these kits, researchers can now label 25 µg or 100 µg of their IgM antibody in as little as 15-30 minutes, ensuring efficient and rapid experimental workflows. These kits offer unparalleled convenience, with less than 30 seconds of hands-on time required, saving valuable laboratory resources.
Mix-n-Stain C Dye IgM Antibody Labelling Kits are available in a selection of eight CF Dyes and FITC. CF Dyes are Biotium’s next-generation fluorescent dyes designed to provide superior brightness and photostability compared to other commercially available fluorescent dyes.
The illustration above shows mouse anti-beta tubulin clone 5H1 (IgM kappa) labelled with CF 488A using Mix-n-Stain IgM labelling, and then was used to stain methanol-fixed HeLa cells at 5 µg/mL (right panel). The conjugate shows comparable staining specificity compared to unlabelled antibody at 1 µg/mL detected using CF 488A Goat Anti-Mouse IgM Mu chain secondary antibody at 2 µg/mL (left panel).
"We are excited to introduce the Mix-n-Stain CF Dye IgM Antibody Labelling Kits to the scientific community," says Dr Lori Roberts, Director of Bioscience at Biotium. "This technology provides a new and simple solution to the longstanding challenge of IgM conjugation. We believe these kits will provide researchers with more flexibility in panel design for immunofluorescence and flow cytometry."
For more information about the Mix-n-Stain CF Dye IgM Antibody Labeling Kits, please visit the product page at https://biotium.com/product/mix-n-stain-cf-dye-igm-antibody-labeling-kits/.