Tried-and-tested ThinCert cell culture inserts from Greiner Bio-One are now available in a new 96-well HTS option, perfect for high-throughput applications. The new product joins the company’s popular 6-, 12- and 24-well options in the range, which is suitable for sophisticated cell and tissue culture applications.
Membrane-based cell culture inserts such as the ThinCert inserts from Greiner Bio-One enable in vitro testing of specific tissue models (eg endothelia and epithelia) in transport, secretion, diffusion, migration, invasion and co-culture studies using air-lift and submersed culture methods.
The existing ThinCert portfolio offers inserts for multiwell plates with a variety of membrane types and pore sizes/densities. Greiner Bio-One’s latest addition is the ThinCert 96-well HTS insert, which supports highly parallelised application. Furthermore, miniaturisation and the reduced use of cells, media and reagents result in much lower costs per assay.
Each ThinCert 96-well HTS insert consists of a 96 well porous membrane plate and a receiver plate. The tissue culture treated PC membrane allows for an ideal exchange of nutrients and substances, creating in vivo-like cultivation conditions to facilitate optimal cell growth, monolayer formation, and tissue differentiation.
Greiner Bio-One offers a unique variant with a special pore configuration that combines excellent permeability and transparency for microscopic monitoring of assays. An alternative with optimum permeability based on a high pore density is also available.
ThinCert HTS inserts with pore sizes of 3μm and 8μm are particularly suited to testing cellular behaviour in motility studies. Their high transparency enables visual monitoring of mechanisms such as cell migration and invasion, which play a key role in physiological and pathological processes, including immune cell migration, the wound-healing process and tumour tissue metastasis.