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Kits for real-time quantitative PCR

GRI, an independent supplier of molecular biology solutions, can now supply KAPA SYBR FAST qPCR kits from Kapa Biosystems, which contain the first DNA polymerase evolved for real-time polymerase chain reaction (PCR) methodology.

The engineered KAPA SYBR DNA polymerase and optimised buffer system provides the highest reaction efficiency, the greatest fluorescence intensity, a broad dynamic range and linearity, and reduced total cycling time. The kit contains a new DNA polymerase that has been produced by a process of molecular evolution. The result is an enzyme designed specifically for quantitative PCR (qPCR) using SYBR Green I dye chemistry.

The KAPA SYBR FAST qPCR kit is available as a ready-to-use 2x master mix containing all the components (except primers and template) required for the amplification and detection of DNA by real-time qPCR. All KAPA SYBR qPCR FAST kits are supplied with antibody-mediated hot start for high specificity and maximum speed, and are ideally suited to gene expression analysis, microarray validation, low-copy gene detection and gene knockdown validation.

www.gri.co.uk

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