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Two-component plates enhance PCR automation

New 4titude FRAMEstar polymerase chain reaction (PCR) plates, now available from Anachem, have a special twocomponent design that combines the advantages of a rigid skirt and deck for reliable robotic handling with thin-walled wells for optimum PCR results. The wells are constructed from virgin polypropylene, which allows efficient heat transfer and provides an inert surface with low binding capabilities for nucleic acids, proteins and other molecules.

The company provides superior products for rapid PCR and small reaction volumes by utilising new injection moulding technology, enabling the wall thickness to be reduced without compromising tube integrity. In comparison with traditional single-piece products, the mechanical stability of the plate is greatly improved by moulding the plate frame in polycarbonate.

Warping, shrinking and thermal distortion are eliminated, so well spacing and position after PCR remain accurate, and liquid handling devices can reliably add or remove the smallest sample quantities from the plate. Furthermore, improved flatness reduces variation of fluorescence signals in optical assays (eg QPCR) and increased seal integrity reduces sample loss.

Anachem
www.anachem.co.uk

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