GE Healthcare has introduced illustra triplePrep, a kit for the rapid and simultaneous isolation of genomic DNA, RNA and total denatured protein from a single undivided sample in less than one hour.
The product is a combination of two spin columns and six optimised buffers for use with mammalian cell culture or animal tissue. High-quality and –yield DNA, RNA and protein can be isolated successfully from a range of tissue types (eg liver, kidney, spleen, brain, lung and intestine) and cell lines (eg HeLa, NIH-3T3, CHO-K1 and HEK-293). Tissue (1–20 mg) and cells (0.3–5 million) can be used for each preparation.
The isolated gDNA, total RNA and total denatured proteins are suitable for various genomic and proteomic applications, including polymerase chain reaction (PCR) methods, restriction enzyme digestion,
sequencing, array CGH, RT-PCR, gene expression microarray, SDS-PAGE, Western blotting, 2D DIGE and LC/MS.