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Kidney research using a single-cell RNA-Seq system

Scientists at the Epigenetics in Human Health and Disease Laboratory, Monash University, Australia, have adopted Dolomite Bio’s Single Cell RNA-Seq system to further their research into animal models of diabetic nephropathy (pictured). Mark Ziemann, research fellow and next-generation sequencing specialist, explained: “Previously, we’ve run bulk RNA sequencing on these animal models, and discovered a lot of variability coming from different cell types in the kidney. Single cell RNA-Seq will help us disentangle the major cell types involved in the disease from those which are not, and determine which cells interact with the immune system.

“Given that this will be an ongoing focus of our research, we thought we’d invest in our own instrument for the benefit of the 65-strong department. We chose Dolomite Bio’s system because it’s open and flexible, offering us the possibility of looking at epigenetic modifications – such as single-cell chromatin immunoprecipitation sequencing and DNA methylation analysis – in the future. In addition, other systems we considered turned out to be a lot more expensive on a per sample basis. We considered developing our own in-house set-up, but committing to reliable chips and pumps from a single supplier ensures that there will be fewer problems down the line, and there’s always someone on the other end of a telephone to help if necessary.”

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Upcoming Events

Pathology Horizons 2024

MacDonald Bath Spa Hotel, Bath
18-20 April, 2024

Diagnostics North East Conference 2024

The Catalyst, Newcastle upon Tyne
19 April, 2024

ECCMID 2024 - European Congress of Clinical Microbiology and Infectious Diseases

Fira Gran Via, 08038 Barcelona, Spain
27-30 April 2024

British Society for Microbial Technology Annual Microbiology Conference

UK Health Security Agency, Colindale, London
2 May 2024

EQA Reports: Interpreting Key Information & Troubleshooting Tips

ONLINE - Zoom
Thursday 16th May 2024

Participants’ Meeting: UK NEQAS Immunology, Immunochemistry & Allergy

Sheffield Hallam University, City Campus, Howard Street, Sheffield
24th May 2024

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

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