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Molecular diagnostics and LAMP technology

Molecular diagnostics have evolved in recent years as an alternative to traditional diagnostic methods such as virus/bacteria culture, microscopy, histopathology-based techniques (ie immunohistochemistry and in situ hybridisation), biochemical, phenotypic or serological analysis. These molecular methods are based on the amplification of a region of the target nucleic acid (either DNA or RNA) and are becoming increasingly popular with end-users.

The polymerase chain reaction (PCR) is considered the molecular diagnostic gold standard; however, there are several disadvantages related to its implementation in laboratories with limited resources, such as the high cost associated with these methods, the need for highly skilled personnel, and the susceptibility to inhibitors commonly present in clinical samples. To counter these disadvantages, molecular methods based on isothermal amplification are becoming established in laboratories worldwide, as these provide similar performance with the additional benefits of reduced costs and minimal training.

Loop-mediated isothermal amplification (LAMP) has the potential to substitute PCR-based methods. Mast Group is incorporating the LAMP technique (Eiken Chemical) in its molecular products (MAST ISOPLEX). Currently, there are two molecular kits already on the market, MAST ISOPLEX DNA Lyo and MAST ISOPLEX DNA Lyo Plus, with two others (MAST ISOPLEX VTEC and MAST ISOPLEX E. coli O157) available soon.

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Upcoming Events

POCT Innovators: The Power to Disrupt Series

Cloth Hall Court, Leeds. UK
20 March, 2024

Interpreting Reports: UK NEQAS Haematology

ONLINE
21st March 2024

USCAP 113th Annual Meeting

Baltimore Convention Center, Baltimore, Maryland, USA
23-28 March, 2024

Pathology Horizons 2024

MacDonald Bath Spa Hotel, Bath
18-20 April, 2024

Diagnostics North East Conference 2024

The Catalyst, Newcastle upon Tyne
19 April, 2024

ECCMID 2024 - European Congress of Clinical Microbiology and Infectious Diseases

Fira Gran Via, 08038 Barcelona, Spain
27-30 April 2024

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

Download the FREE Pathology In Practice app from your device's App store

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