Sphere Fluidics has introduced the Cyto-Cellect Human IgG Kappa Detection Kit, to be used in conjunction with the Cyto-Mine Single Cell Analysis System for rapid and cost-effective measurement of antibody class and productivity in cell line development.
The Cyto-Cellect Human IgGK Detection Kit has been developed to provide Cyto-Mine users with an easy-to-use, standardised assay format for the detection of secreted human antibodies in picodroplets. The sensitive FRET-based assay rapidly detects the presence of human IgG with kappa light chain after secretion from encapsulated cells, to characterise and measure antibody production. Rapid detection of IgG enables high-throughput identification and selection of cells with the highest productivity for more efficient cell-line development.
With optimised ratios of Sphere Fluidics’ Human IgGK Detection probes, the Cyto-Cellect Human IgGK Detection Kit offers a sensitive and robust assay ideal for streamlining cell isolation and titre determination. The complete kit has been manufactured without the use of animal-derived materials, offering an animal origin-free reagent kit that provides confidence in the reproducibility and consistency of the assay and conforms to regulatory requirements in cell-line development.
Dr Zoe Nilsson (Global Product Marketing Manager, Sphere Fluidics), said: “Our expert team has applied extensive knowledge of assay requirements to develop the Cyto-Cellect Human IgGK Detection Kit, offering users a sensitive, yet robust assay solution for the Cyto-Mine platform. The addition of this assay-ready kit to Sphere Fluidics’ product portfolio provides a rapid, yet cost-effective solution to support and reduce cell line development timelines.”