The website of Pathology In Practice Magazine

Loop-mediated isothermal amplification: from brucellosis to SARS-CoV-2

The development of new nucleic acid amplification techniques is bringing molecular diagnostics closer to the patient for faster results and improved patient management. A prime example of this latest near-patient capability is LAMP technology.

Guidelines for the diagnosis and management of many conditions often now require a molecular assay result, involving the detection of a specific nucleic acid sequence in a patient sample. The molecular result may be qualitative (eg for confirmation of the disease or condition) or quantitative (eg to measure viral loads in the monitoring of patients or to assess treatment success).

The detection of a specific nucleic acid sequence in a biological sample that contains huge amounts of DNA and/or RNA is extremely challenging. Therefore, nucleic acid amplification techniques such as the polymerase chain reaction (PCR) have been used to make multiple copies of the target nucleic acid sequence, allowing it to be detectable against background nucleic acids.

The special equipment and expertise required for molecular diagnostics previously meant that assays had to be performed in central, specialised molecular laboratories. Now, however, with the ad

Log in or register FREE to read the rest

This story is Premium Content and is only available to registered users. Please log in at the top of the page to view the full text. If you don't already have an account, please register with us completely free of charge.
Register

Upcoming Events

Every Minute Counts: a SHOT, BBTS and NBTC joint webinar

Online
3 December 2021

IBMS Biomedical Science Congress 2022

International Convention Centre, Birmingham
14-17 March 2022

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

Download the FREE Pathology In Practice app from your device's App store

Upcoming Events

Every Minute Counts: a SHOT, BBTS and NBTC joint webinar

Online
3 December 2021

IBMS Biomedical Science Congress 2022

International Convention Centre, Birmingham
14-17 March 2022

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

Download the FREE Pathology In Practice app from your device's App store

Step Communications Ltd, Step House, North Farm Road, Tunbridge Wells, Kent TN2 3DR
Tel: 01892 779999 Fax: 01892 616177
www.step-communications.com
© 2021 Step Communications Ltd. Registered in England. Registration Number 3893025