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Loop-mediated isothermal amplification: from brucellosis to SARS-CoV-2

The development of new nucleic acid amplification techniques is bringing molecular diagnostics closer to the patient for faster results and improved patient management. A prime example of this latest near-patient capability is LAMP technology.

Guidelines for the diagnosis and management of many conditions often now require a molecular assay result, involving the detection of a specific nucleic acid sequence in a patient sample. The molecular result may be qualitative (eg for confirmation of the disease or condition) or quantitative (eg to measure viral loads in the monitoring of patients or to assess treatment success).

The detection of a specific nucleic acid sequence in a biological sample that contains huge amounts of DNA and/or RNA is extremely challenging. Therefore, nucleic acid amplification techniques such as the polymerase chain reaction (PCR) have been used to make multiple copies of the target nucleic acid sequence, allowing it to be detectable against background nucleic acids.

The special equipment and expertise required for molecular diagnostics previously meant that assays had to be performed in central, specialised molecular laboratories. Now, however, with the ad

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analytica 2022

Messe München, Germany
June 21-24, 2022

The Genomic and Microbiology Revolution: In Technology We Trust?

Royal Air Force Museum, Hendon, London
19 July 2022

Arab Lab Live 2022

Dubai International Convention & Exhibition Centre Dubai World Trade Centre - Trade Centre - Trade Centre 2 - Dubai - United Arab Emirates
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14 - 17 November 2022

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

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