Various immunological methods have been used to diagnose coeliac disease. Here, Charlene Porter, Irene Gray and David Wilson assess an alternative platform for the detection of anti-tTG.
(Image modified from reference 3 with kind permission)
Coeliac disease (CD) is an autoimmune condition resulting from an adverse reaction to gluten, which is a protein found in wheat, barley and rye. The prevalence of CD is approximately 0.5–1% of the general population and is strongly associated with the human leucocyte antigen (HLA)-linked genes DQ2 and DQ8.1,2
Currently, the authors’ laboratory in Aberdeen uses an enzyme-linked immunoassay (ELiA) to detect anti-tissue transglutaminase (anti-tTG) to aid in the diagnosis of CD. Initial presentations are also confirmed by endomysial antibody (anti-EMA) testing.
This short study aims to discover whether or not a new anti-tTG chemiluminescence (CIA) assay would provide better correlation with anti-EMA results when compared to the department’s current anti-tTG ELiA method.
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