Software to reduce variability in ELISA biomarker tests

A new computational approach has been developed to reduce variability in common research biomarker tests, a promising step in improving the ability of biomedical researchers and basic scientists to reproduce data and facilitate more consistent results across laboratories and long-term projects. Researchers from Boston Medical Center (BMC) and Boston University School of Medicine (BUSM), developed a new software program, ELISAtools, which provides a stable platform to

Enzyme-linked immunosorbent assay (ELISA) tests are used globally across clinical, biomedical and basic research fields to measure biomarkers in a range of mediums including blood, plasma and urine. Clinical ELISA test kits used in the hospital setting are regulated to ensure tight quality control boundaries for accuracy and consistency. However, the hundreds of commercially available research-use-only ELISA test kits are not regulated, which often leads to noticeable variability in results over time, between testing kits, and across different laboratories.

 

The BMC-BUSM research team unexpectedly encountered high variability from one ELISA test kit during a project for the National Cancer Institute measuring thrombosis and inflammation biomarkers in the plasma of cancer subjects and healthy donors.  After the first year of the project, they realised the data was changing significantly as they received different shipments of the kit from the manufacturer. After a thorough examination, they determined differences in the ELISA kit was causing the issue. They had research data from over 400 patient samples that could not be compared due to these differences in the ELISA kits.  To solve this problem, the research team created the ELISAtools software program to reduce future variability in test results.

www.bmc.org

 

Other news

Upcoming Events

HUCBMS 2019

Technological University Dublin
3-4 September 2019

The Oxford Haematology in Obstetrics Postgraduate Course

St Edmund Hall, Queen's Lane, Oxford OX1 4AR
9-11 September 2019

IBMS Biomedical Science Congress

ICC, Birmingham
22-25 September 2019

Lab Innovations

NEC, Birmingham (Hall 12)
30 & 31 October 2019

Latest Issue

Pathology In Practice

Pathology In Practice

Jun 2019

Recent advances in cancer diagnostics

Register now to apply for regular copies of Pathology In Practice and free access to premium content, as well as our regular newsletters.